Homozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth. true B（1〜3か月） 1997年発表、糸原重美、伊藤　剛、京都大学ウイルス研究所 RBRC00398 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 272, 22389-22392 (1997). B (1-3 months) E14 [129P2/OlaHsd] ゲラチネースA（メタロプロテイネース2型）を完全に欠失する。軽度の発育不全を示す。 Cardiovascular Research Developmental Biology Research Developed by Takeshi Itoh and Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1997. The mutant mice were backcrossed to C57BL/6. This strain was generated by gene targeting of gelatinase A (Mmp2, metalloproteinase 2). Homozygous mutant mice are completely devoid of gelatinase A. 5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth. GelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito GelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 糸原　重美 RIKEN,BSI <a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/00398_GB.pdf">Genetic Background</a> <a href='https://brc.riken.jp/mus/pcr00398'>Genotyping protocol -PCR-</a> 理化学研究所脳科学総合研究センター B6.129P2-Mmp2<tm1Ito>/ItoRbrc B6.129P2-Mmp2<tm1Ito>/ItoRbrc 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Biol. Chem., 272, 22389-22392 (1997). ShigeYoshi ITOHARA mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse GelA genomic DNA